| Main Title |
Use of Prolonged Treatment and the Fluctuation Test to Detect Mutations in Human Fibroblasts Treated with Methyl Methanesulfonate. |
| Author |
Huang, Shiu L. ;
Biddix, Gwyn ;
Waters, Michael D. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1982 |
| Report Number |
EPA-600/J-82-302; |
| Stock Number |
PB83-189563 |
| Additional Subjects |
Toxicology ;
Humans ;
Reprints ;
Methyl methanesulfonate ;
Fibroblasts ;
Mutagenesis ;
Fluctuation test
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB83-189563 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
The methyl methanesulfonate (MMS) induction of 6-thioguanine-resistant (6TG) mutants in non-synchronized human fibroblasts has been quantitatively characterized. A 24-h MMS treated failure to induce mutations in non-synchronized cultures. However, exposure of growing cells to MMS for 3 days caused a significant increase in the mutation frequencies. The mutations induction was dose-dependent. The fluctuation test was also useful in detecting the mutagenic activity of MMS in human fibroblasts. Prolonged treatment of cultures made the human cell mutagenesis testing system more sensitive. |
