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Main Title Developmental Toxicity of Inhaled Methanol in the CD-1 Mouse, with Application of Quantitative Dose-Response Modeling for Estimation of Benchmark Doses.
Author Rogers, J. M. ; Mole, M. L. ; Chernoff, N. ; Barbee, B. D. ; Turner, C. I. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Perinatal Toxicology Branch.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/350;
Stock Number PB93-106979
Additional Subjects Toxicity ; Methanol ; Teratogenic compounds ; Cleft palate ; Mice ; Dose-response relationships ; Congenital abnormalities ; Fetus ; Maternal-fetal exchange ;
Library Call Number Additional Info Location Last
NTIS  PB93-106979 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 37p
Pregnant CD-1 mice were exposed to 1,000, 2,000, 5,000, 7,500, 10,000 or 15,000 ppm on methanol for 7 hr/day on days 6-15 of gestation. On day 17 of gestation, remaining mice were weighed, killed and the gravid uterus was removed. Numbers of implantation sites, live and dead fetuses and resorptions were counted, and fetuses were examined externally and weighed as a litter. Half of each litter was examined for skeletal morphology and the other half of each litter was examined for internal soft tissue anomalies using a freehand scalpel dissection. Significant increases in the incidence of exencephaly and cleft palate were observed at 5,000 ppm and above, increased postimplantation mortality at 7,500 ppm and above (including an increasing incidence of full-litter resorption), and reduced fetal weight at 10,000 ppm and above. A dose-related increase in cervical ribs or ossification sites lateral to the seventh cervical vertebra was significant at 2,000 ppm and above. The results of the study indicate that inhaled methanol is developmentally toxic in the mouse at exposure levels which were not maternally toxic. Litters of pregnant mice gavaged orally with 4 g methanol/kg displayed developmental toxic effects similar to those seen in the 10,000 ppm methanol exposure group.