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RECORD NUMBER: 2 OF 2

Main Title RNA Polymerase-Binding and Transcription Initiation Sites Upstream of the Methyl Reductase Operon of 'Methanococcus vannielii'.
Author Thomm, M. ; Sherf, B. A. ; Reeve, J. N. ;
CORP Author Ohio State Univ., Columbus. Dept. of Microbiology. ;Regensburg Univ. (Germany, F.R.). Lehrstuhl fuer Mikrobiologie.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;North Atlantic Treaty Organization, Brussels (Belgium).
Publisher c1988
Year Published 1988
Report Number EPA-R-812774; EPA/600/J-88/371;
Stock Number PB90-100512
Additional Subjects Micrococcaceae ; Electrophoresis ; Binding ; Deoxyribonucleic acids ; Reprints ; Genetic transcription ; RNA polymerases ; Exonucleases ; Codon ; Methonococcus vannielii
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB90-100512 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
Abstract
RNA polymerase, purified from Methanococcus vannielii, was shown by exonuclease III footprinting to bind to a 49-base-pair (bp) region of DNA in the intergenic region upstream of mcrB. S1 nuclease protection experiments demonstrated that transcription initiation in vivo occurs within this region at 32 or 33 bp 5' to the ATG translation initiation codon of mcrB and 19 or 20 bp 3' to a TATA box. (Copyright 1988, American Society for Microbiology.)