Surveys of fish community status were conducted in summer 1987 in 49 lakes in Subregion 2B, the Upper Peninsula of Michigan, as part of Phase II of the Eastern Lake Survey. Lake selection involved a variable probability sampling design. Fish communities were surveyed using gill nets, trap nets, beach seines, and angling. Fish were collected in 47 of the 49 lakes surveyed. Extrapolation of the results to the Eastern Lake Survey Phase II target population suggests that 99.4% of the lakes in the area support fish. For the 49 survey lakes, the number of fish species caught per lake (species richness) was lower in seepage lakes (without inlets or outlets) than in non-seepage lakes and was also lower in lakes with lower pH, ANC, calcium (and other base cations), DOC, and silica and with higher levels of extractable aluminum. In contrast to the large number of variables associated with fish presence/absence and species richness, variations in the numbers of fish caught and catch per unit effort among lakes appeared to be independent of lake characteristics.