Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Missouri River Basin Sterol Assay Project Report. Coprostanol, A Positive Marker of Domestic and Run-off Pollution. Sterol Assay of Wastewater Plant Effluents and Surface Waters of the Lower Main Stem Missouri.
Author Tabak, Henry H. ; Bunch., Robert L. ;
CORP Author Federal Water Quality Administration, Cincinnati, Ohio. Advanced Waste Treatment Research Lab.
Year Published 1970
Stock Number PB-256 930
Additional Subjects Sewage ; Runoff ; Sterols ; Effluents ; Missouri River ; Sampling ; Concentration(Composition) ; Membranes ; Filters ; Gas chromatography ; Extraction ; Thin layer chromatography ; Marking ; Water quality ; Cholestanols ; Coprostanols ; Water quality data
Library Call Number Additional Info Location Last
NTIS  PB-256 930 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 18p
This study was undertaken to estimate the extent of human and warm-blooded animal fecal pollution and to pinpoint the main sources of this pollution in the Missouri River. The survey covered 28 sampling stations in the Sioux City, Omaha, St. Joseph and Kansas City area. The analytical method used for the estimation of the coprostanol in the river waters and in the wastewaters was based on the methodology which was previously developed by Murtaugh & Bunch. There was one major change in that a new packing was used for the gas-liquid chromatography column which permitted the free sterols to be run. This eliminated the need for making the trimethylsilyl ether derivatives. The procedure consisted of hexane extraction, mild alkali-alcohol hydrolysis of esters and conjugates to free sterols, cleanup by thin-layer chromatography (TLC) and quantitative measurement by gas-liquid chromatography (GLC). Simultaneously with the assay for the fecal sterol the number of fecal coliforms were determined utilizing the membrane filter technology.