Record Display for the EPA National Library Catalog


Main Title In vitro Study of Embryotoxic and Dysmorphogenic Effects of Mercuric Chloride and Methylmercury Chloride in the Rat.
Author Kitchin, K. T. ; Ebron, M. T. ; Svendsgaard, D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA-600/J-84-015;
Stock Number PB84-197581
Additional Subjects Toxicology ; Rats ; Laboratory animals ; In vitro analysis ; Reprints ; Mercuric chloride ; Methylmercury chloride ; Dysmorphogeny ; Embryotoxicity
Library Call Number Additional Info Location Last
NTIS  PB84-197581 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Rat-embryo explants removed on day 10.5 of gestation were cultured for 48 hr in various concentrations of HgCl2 CH3HgCl or glutathione. Dose-related dysmorhogenesis and growth retardation occurred with increasing concentrations of HgCl2 (1-10 micro M). Increasing concentrations of CH3HgCl (3-100 micro M) produced a similar pattern of embryonic effects. The rat-serum incubation medium had no detectable level of reduced glutathione (0.02 mM) and only 0.12 mM total sulphydryl groups. Reduced glutathione (60-300 micro M) added to the incubation medium was relatively non-toxic. The addition of exogenous glutathione to culture medium containing HgCl2 partially antagonized the embryonic growth retardation and prevented most of the embryolethality observed in cultures to which only HgCl2 had been added.