Record Display for the EPA National Library Catalog


Main Title Isolation of a Cytochrome P-450 Structural Gene from 'Saccharomyces cerevisiae'.
Author Kalb, V. F. ; Loper, J. C. ; Dey, C. R. ; Woods, C. W. ; Sutter, T. R. ;
CORP Author Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1986
Year Published 1986
Report Number EPA-R-810605; EPA/600/J-93/330;
Stock Number PB93-229482
Additional Subjects Cytochrome P450 ; Structural genes ; Saccharomyces cerevisiae ; Drug resistance ; Ketoconazole ; Genetic vectors ; Plasmids ; Open reading frames ; Heme ; Molecular cloning ; Antibodies ; Recombinant DNA ; Genetic translation ; Genetic transformation ; Molecular sequence data ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-229482 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
A Saccharomyces cerevisiae host with an S. cerevisiae genomic library contained in the shuttle vector YEp24 was transformed and the resultant transformants were screened for resistance to ketoconazole (Kc), an inhibitor of the cytochrome P-450 (P-450) enzyme lanosterol 14 alpha-demethylase. Two plasmids were isolated which transformed yeast to both increased resistance to Kc and increased levels of total P-450. Hybrid-selection and immunoprecipitation experiments showed that these plasmids, pVK1 and pVK2, contained the structural gene for an S. cerevisiae P-450. This conclusion was confirmed by the nucleotide sequence of a portion of pVK2, which revealed an open reading frame encoding a characteristic P-450 heme-binding region.