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Main Title Isolation and Characterization of the Alkane-Inducible NADPH-Cytochrome P-450 Oxidoreductase Gene from 'Candida tropicalis'.
Author Sutter, T. R. ; Sanglard, D. ; Loper, J. C. ;
CORP Author Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-93/327;
Stock Number PB93-229458
Additional Subjects Enzyme induction ; NADPH cytochrome reductase ; Alkanes ; Flavoproteins ; Messenger RNA ; Amino acid sequence ; Sequence alignment ; Reprints ; Candida tropicalis
Library Call Number Additional Info Location Last
NTIS  PB93-229458 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
The gene coding for the Candida tropicalis NADPH-cytochrome P-450 oxidoreductase was isolated by immunoscreening of a C. tropicalis (lambda)gt11 expression library and colony hybridization of a C. tropicalis genomic library. The C. tropicalis CPR gene produces a 2.35-kilobase mRNA transcript, levels of which were shown to be increased 16-fold in cells grown on tetradecane relative to cells grown on glucose as the sole carbon source. A 3-kilobase DNA fragment was sequenced, including 554 and 397 base pairs of 5'- and 3'-noncoding sequence, respectively. A single open reading frame of 2040 base pairs was identified and predicts a 76,683-Da polypeptide of 680 amino acid residues. The deduced C. tropicalis CPR amino acid sequence was compared with each of the CPR sequences reported from other organisms and invariant residues were identified. (Copyright (c) 1990 by The American Society for Biochemistry and Molecular Biology, Inc.)