| Main Title |
Optimization of the Hamilton-Thorn Computerized Sperm Motility Analysis System for Use with Rat Spermatozoa in Toxicological Studies. |
| Author |
Slott, V. L. ;
Suarez, J. D. ;
Poss, P. M. ;
Linder, R. E. ;
Strader, L. F. ;
|
| CORP Author |
ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch. |
| Publisher |
c1993 |
| Year Published |
1993 |
| Report Number |
EPA-68-D2-0056; EPA/600/J-94/068; |
| Stock Number |
PB94-141603 |
| Additional Subjects |
Sperm motility ;
Toxicology ;
Spermatozoa ;
Computer simulation ;
Rats ;
Reproducibility of results ;
Reproduction(Biology) ;
Risk assessment ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB94-141603 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
To optimize the Hamilton-Thorn Motility Analyzer (HTM; Hamilton-Thorn Research, Beverly, MA) for use in reproductive toxicology studies with rat spermatozoa, the accuracy and precision of the instrument were assessed under a variety of instrument settings. Videotapes of both fast- and slow-swimming sperm were analyzed repeatedly to obtain data across a range of sperm velocities as might be encountered as a consequence of exposure to reproductive toxicants. Acquisition rates were varied across the HTM menu choices as were the number of frames analyzed at each framing rate. For fast-swimming samples (mean straight-line velocity (VSL) approximately 130 micrometers/sec) generally good agreement between computer-assisted sperm analysis (CASA) and manually obtained data was found for percentage of motile sperm and straight-line velocity; i.e., CASA values were within 10% of manual values for most frame/rate combinations. (Copyright (c) 1993 by the Society of Toxicology.) |
