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Main Title Methods for studying bacterial gene transfer in soil by conjugation and transduction /
Author Stotzky, G. ; Devanas, M. A. ; Zeph, L. R.
Other Authors
Author Title of a Work
Devanas, Monica A.
Zeph, Lawrence R.
CORP Author Corvallis Environmental Research Lab., OR. ;New York Univ., NY. Lab. of Microbial Ecology.
Publisher Laboratory of Microbial Ecology, Department of Biology, New York University,
Year Published 1989
Report Number EPA/600/3-89/042;
Stock Number PB89-195044
OCLC Number 754605885
Subjects Environmental Microbiology ; Gene Transfer Techniques
Additional Subjects Genetic engineering ; Bacteria ; Soil microbiology ; Molecular biology ; Tests ; Manuals ; Genetic conjugation ; Transfection ; Recombinant DNA ; Risk assessment
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EKCD  EPA/600/3-89/042 CEMM/GEMMD Library/Gulf Breeze,FL 07/17/2018
NTIS  PB89-195044 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 1 volume (various pagings) : illustrations ; 28 cm
The document provides a series of protocols by which a trained technician can conduct studies on the transfer of genetic information by conjugation or transduction in soil, with emphasis on bacteria containing recombinant DNA. The level of the document is geared to technicians with some background and experience in standard laboratory methods used in microbiology but who have limited knowledge of, and experience in, bacterial genetics, molecular biology, and microbial ecology, especially in soil. Many of the specific techniques described in the document for studying gene transfer in soil were developed by the authors during the past few years. The primary motivation for the studies was the need for information on the survival of, and gene transfer by, genetically engineered bacteria that could be used in risk assessment of the release of genetically engineered microorganisms to the environment.
"EPA-600/3-89/042, April 1989"--Cover. "Research and Development"--Cover. Includes bibliographical references (pages 1-26). U.S. Environmental Protection Agency's Environmental Research Laboratory,