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RECORD NUMBER: 7 OF 12

Main Title Laboratory Culture of Gametophytic Stages of the Marine Microalgae 'Champia parvula' (Rhodophyta) and 'Laminaria saccharina' (Phaeophyta).
Author Steele, R. L. ; Thursby, G. B. ;
CORP Author Environmental Research Lab., Narragansett, RI. ;Rhode Island Univ., Kingston. Dept. of Botany.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/447 ;ERLN-840;
Stock Number PB90-126624
Additional Subjects Toxicity ; Algae ; Water pollution ; Aquaculture ; Temperature ; Sea water ; Growth ; Reprints ; Champia parvula ; Red algae ; Laminaria saccharina ; Brown algae
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB90-126624 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Abstract
Methods to help characterize the toxicity of effluent discharges within the National Pollutant Discharge Elimination System have been developed for the marine red alga Champia parvula and are currently being developed for the brown alga Laminaria saccharina. These methods require maintaining male and female gametophytes in laboratory cultures. Aseptic techniques are recommended even though the cultures are not intended to be axenic; the purpose is to guard against microalgal contamination. C. parvula can be grown at room temperature, but L. saccharina requires a controlled temperature chamber (12-15 C). Both species can be cultured successfully in either natural or artificial seawater.