Record Display for the EPA National Library Catalog


Main Title Guidelines for conducting early life stage toxicity tests with Japanese medaka (Oryzias latipes) /
Author Benoit, Duane A. ; Benoit, D. A. ; Holcombe, G. W. ; Spehar, R. L.
Other Authors
Author Title of a Work
Holcombe, Gary W.
Spehar, Robert L.
CORP Author Environmental Research Lab.-Duluth, MN.
Publisher U.S. Environmental Protection Agency,
Year Published 1991
Report Number EPA/600/3-91/063
Stock Number PB92-137488
OCLC Number 25110349
Subjects Oryzias latipes ; Toxicity testing
Additional Subjects Water pollution effects(Animals) ; Oryzias ; Life cycles ; Toxic substances ; Embryo ; Test methods ; Manuals ; Temperature ; Exposure ; Light ; Oxygen ; Guidelines
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EHBD  EPA/600/3-91/063 CEMM/ACESD Library/Narragansett,RI 03/16/2007
EJED  EPA-600/3-91-063 OCSPP Chemical Library/Washington,DC 07/24/1992
EKCD  EPA/600/3-91/063 CEMM/GEMMD Library/Gulf Breeze,FL 07/17/2018
ELBD ARCHIVE EPA 600-3-91-063 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023 DISPERSAL
ELDD  EPA/600/3-91/063 CCTE/GLTED Library/Duluth,MN 05/30/1992
EMBD  EPA/600/3-91/063 NRMRL/GWERD Library/Ada,OK 05/01/1992
ERAD  EPA 600/3-91/063 Region 9 Library/San Francisco,CA 11/27/1992
ESAD  EPA 600-3-91-063 Region 10 Library/Seattle,WA 03/23/2010
NTIS  PB92-137488 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 25 pages : illustrations ; 28 cm
The manual represents a procedural guide for conducting embryo-larval early life stage (ELS) toxicity tests with Japanese medaka (Oryzias latipes). These procedures are based upon evaluation of published papers and recent methods development work conducted at a laboratory in Duluth. The published papers are referenced in the appropriate places throughout the text of the report. If more detailed information on test apparatus or specific biological and chemical methods is desired, the reader is encouraged to study the reference material or contact one of the authors of the manual. All routine methods not covered in the procedure (physical and chemical determinations), should be followed as described in Standard Methods for the Examination of Water and Wastewater (3). It is suggested that those routines dealing with handling of fish not covered in the procedure should be followed as described by ASTM (4).
"EPA/600/3-91/063." "December 1991." "Environmental Research Laboratory-Duluth, Office of Research and Development." Includes bibliographical references.