Record Display for the EPA National Library Catalog


Main Title Determination of Acrylamide in Rat Serum and Sciatic Nerve by Gas Chromatography-Electron-Capture Detection.
Author Raymer, J. H. ; Sparacino, C. M. ; Velez, G. R. ; Padilla, S. ; MacPhail, R. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Research Triangle Inst., Research Triangle Park, NC. Analytical and Chemical Sciences Unit.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-94/070;
Stock Number PB94-141587
Additional Subjects Blood ; Sciatic nerve ; Acrylamides ; Chemical analysis ; Gas chromatography ; Electrons ; Kinetics ; Tissues(Biology) ; Mass spectroscopy ; Dose-response relationships ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-141587 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
A modified method for the derivatization and determination of acrylamide as 2-bromopropenamide by gas chromatography-electron-capture detection was developed and applied to serum and sciatic nerve from rats. The method was accurate and precise over the calibration range 2.24-7.47 micrograms/ml in serum diluted 1:125 and 4-122 micrograms/g in sciatic nerve homogenate (5 mg/ml). Limits of detection were estimated to be 1,200 ng/ml in undiluted serum and 3 micrograms/g in intact sciatic nerve. The use of less dilute samples to allow for lower limits of detection appears feasible. The time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 h for serum and sciatic nerve, respectively. A dose-effect relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum (micrograms/ml) were approximately the same as in sciatic nerve (micrograms/g). (Copyright (c) 1993 Elsevier Science Publishers B.V.)