Record Display for the EPA National Library Catalog


Main Title Concentration of Poliovirus from Tap Water Using Positively Charged Microporous Filters.
Author Sobsey, Mark D. ; Jones, Baxter L. ;
CORP Author North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;Health Effects Research Lab., Cincinnati, OH.
Year Published 1978
Report Number EPA-R-804218; EPA-600/J-79-140;
Stock Number PB82-118258
Additional Subjects Water treatment ; Filters ; Polioviruses ; Potable water ; Viruses ; Performance evaluation ; Comparisons ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB82-118258 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Microporous filters that are more electropositive than the negatively charged filters currently used for virus concentration from water by filter adsorption-elution methods were evaluated for poliovirus recovery from tap water. Zeta Plus filters composed of diatomaceous earth-cellulose-'charge-modified' resin mixtures and having a net positive charge of up to pH 5 to 6 efficiently adsorbed poliovirus from tap water at ambient pH levels 7.0 to 7.5 without added multivalent cation salts. The adsorbed viruses were eluted with glycine-NaOH, pH 9.5 to 11.5. Electropositive asbestos-cellulose filters efficiently adsorbed poliovirus from tap water without added multivalent cation salts between pH 3.5 and 9.0, and the absorbed viruses could be eluted with 3% beef extract, pH 9, but not with pH 9.5 to 11.5 glycine-NaOH. Under water quality conditions in which poliovirus recoveries from large volumes of water were less than 5% with conventional negatively charged filters and standard methods, recoveries with Zeta Plus filters averaged 64 and 22.5% for one- and two-stage concentration procedures, respectively. Electropositive filters appear to offer distinct advantages over conventional negatively charged filters for concentrating enteric viruses from water, and their behavior tends to confirm the importance of electrostatic forces in virus recovery from water by microporous filter adsorption-elution methods.