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Main Title Computer-assisted sperm analysis epididymal sperm motility using the Hamilton-Thorn Motility Analyzer /
Author Slott, Valerie L. ; Perreault., S. D.
Other Authors
Author Title of a Work
Perreault, Sally D.
CORP Author ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Health Effects Research Laboratory,
Year Published 1992
Report Number EPA/600/A-92/070; EPA-68-02-4450
Stock Number PB92-165000
Subjects Hamsters--Research
Additional Subjects Sperm motility ; Epididymis ; Rats ; Toxicology ; Reproduction(Biology) ; Computer-assisted image analysis ; Culture media ; Videotape recording ; CASA(Computer-assisted sperm motion analysis)
Library Call Number Additional Info Location Last
NTIS  PB92-165000 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 25 pages : illustrations ; 28 cm
Computer-assisted sperm motion analysis (CASA) can provide a comprehensive evaluation of sperm motility in an efficient and objective manner. The inclusion of CASA in reproductive toxicology studies on male rodents results in a more thorough characterization of adverse effects on spermatogenesis than the use of sperm counts or fertility data alone. Compared to human sperm, which CASA systems were developed for, rat sperm are considerably larger and the motion of the tracked images contains an added flagellar component due to the inclusion of the midpiece and tail in the digitized image. These differences must be taken into account when adapting CASA technology to assess rat sperm. Included in the chapter are techniques and recommendations for obtaining and maintaining quality sperm samples from the rat cauda epididymis, as well as procedures for handling, videotaping and analyzing the motility of these samples using the HTM-2000 Motility Analyzer (Hamilton-Thorn Research, Beverly, MA). Additionally, causes and remedies of possible sperm tracking errors for the machine and the utility of CASA technology are discussed.
Cover title. Sponsoring agency: Health Effects Research Lab., Research Triangle Park, NC. PB92-165000. Includes bibliographical references. Microfiche.