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RECORD NUMBER: 7 OF 7

OLS Field Name OLS Field Data
Main Title Metabolism of Benzo(a)pyrene in Monolayer Cultures of Human Bronchial Epithelial Cells from a Series of Donors.
Author Siegfried, J. M. ; Rudo, K. ; Bryant, B. J. ; Ellis, S. ; Mass, M. J. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA-68-02-4031; EPA/600/J-86/223;
Stock Number PB87-171054
Additional Subjects Bronchi ; Cells(Biology) ; Damage ; Carcinogens ; Humans ; Epithelium ; Tissues(Biology) ; Metabolism ; Malignant neoplasms ; Smoking ; Toxicity ; Reprints ; Benzopyrene
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB87-171054 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 6p
Abstract
Benzo(a)pyrene (B(a)P) metabolism was measured in monolayer cultures of human bronchial epithelial cells derived from eighteen specimens of explanted tissue. Bronchial epithelial cells converted B(a)P to dihydrodiols, phenols, quinione derivatives, and polyhydroxylated forms. Sulfate and glucuronide conjugates of B(a)P metabolites were also detected. Both total metabolism and distribution of metabolites showed a 10-fold or greater variation in cultures from different specimens. When the data were divided according to smoking status, however, no differences in total metabolism, extent of conjugation, or distribution of metabolites could be demonstrated between the two groups. The results suggest that human bronchial epithial cells which are newly grown from explanted tissue of smokers in culture do not demonstrate enzymatic induction.