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RECORD NUMBER: 6 OF 6

Main Title Selection of a 'Pseudomonas cepacia' Strain Constitutive for the Degradation of Trichloroethylene.
Author Shields, M. S. ; Reagin, M. J. ;
CORP Author Technical Resources, Inc., Gulf Breeze, FL. ;University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1992
Year Published 1992
Report Number EPA-68-03-3479; EPA/600/J-93/068 ; CONTRIB-790
Stock Number PB93-169027
Additional Subjects Biodeterioration ; Trichloroethylene ; Growth ; Toluene ; Phenols ; DNA insertion elements ; Mutation ; Chlorine organic compounds ; Reprints ; Pseudomonas cepacia
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NTIS  PB93-169027 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Abstract
Tn5 insertion mutants of Pseudomonas cepacia G4 that were unable to degrade trichloroethylene (TCE), toluene, or phenol or to transform m-trifluoromethyl phenol (TFMP) to 7,7,7-trifluoro-2-hydroxy-6-oxo-2,4-heptadienoic acid (TFHA) were produced. Spontaneous reversion to growth on phenol or toluene as the sole source of carbon was observed in one mutant strain, G4 5223, at a frequency of about .0004 per generation. One such revertant, G4 5223-PR1, metabolized TFMP to TFHA and degraded TCE. Unlike wild-type G4, G4 5223-PR1 constitutively metabolized both TFMP and TCE without aromatic induction. G4 5223-PR1 also degraded cis-1,2-dichloroethylene, trans-1,2-dichloroethylene, and 1,1-dichloroethylene and oxidized naphthalene to alpha naphthol constitutively. G4 5223-PR1 exhibited a slight retardation in growth rate at TCE concentrations of greater than or equal to 530 microM, whereas G4 (which was unable to metabolize TCE under the same noninducing growth conditions) remained unaffected. The constitutive degradative phenotype of G4 5223-PR1 was completely stable through 100 generations of nonselective growth. (Copyright (c) 1992, American Society for Microbiology.)