Record Display for the EPA National Library Catalog


Main Title Degradation of the Chlorinated Phenoxyacetate Herbicides 2,4-Dichlorophenoxyacetic Acid and 2,4,5-Trichlorophenoxyacetic Acid by Pure and Mixed Bacterial Cultures.
Author Haugland, R. A. ; Schlemm, D. J. ; Lyons, R. P. ; Sferra, P. R. ; Chakrabarty, A. M. ;
CORP Author Illinois Univ. at the Medical Center, Chicago. Dept. of Microbiology and Immunology.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;National Inst. of Environmental Health Sciences, Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number PHS-ES-04050-04; EPA/600/J-93/236;
Stock Number PB93-205185
Additional Subjects Biodeterioration ; Herbicides ; 2-4-dichlorophenoxyacetic acid ; 2-4-5-trichlorophenoxyacetic acid ; Alcaligenes ; Pseudomonas cepacia ; Growth ; Liquid chromatography ; Transfection ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-205185 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Combined cell suspensions of the 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) metabolizing organism Pseudomonas cepacia AC1100, and the 2,4-dichlorophenoxyacetic acid (2,4-D)-metabolizing organism Alcaligenes eutrophus JMP134 were shown to effectively degrade either of these compounds provided as single substrates. These combined cell suspensions, however, poorly degraded mixtures of the two compounds provided at the same concentrations. Growth and viability studies revealed that such mixtures of 2,4-D and 2,4,5-T were toxic to AC1100 alone and to combinations of AC1100 and JMP134. High-pressure liquid chromatography analyses of culture supernatants of AC1100 incubated with 2,4-D and 2,4,5-T revealed the accumulation of chlorohydroquinone as an apparent dead-end catabolite of 2,4-D and the subsequent accumulation of both 2,4-dichlorophenol and 2,4,5-trichlorophenol. JMP134 cells incubated in the same medium did not catabolize 2,4,5-T and were also inhibited in initiating 2,4-D catabolism. A new derivative of strain AC1100 was constructed by the transfer into the organism of the 2,4-D-degradative plasmid pJP4 from strain JMP134. The new strain, designated RHJ1, was shown to efficiently degrade mixtures of 2,4-D and 2,4,5-T through the simultaneous metabolism of these compounds. (Copyright (c) 1990, American Society for Microbiology.)