| Main Title |
Molecular cloning : a laboratory manual / |
| Author |
Sambrook, Joseph.
|
| Other Authors |
|
| Publisher |
Cold Spring Harbor Laboratory Press, |
| Year Published |
2001 |
| OCLC Number |
45015638 |
| ISBN |
0879695773 (paper) 9780879695774 (paper) 0879695765 (cloth) 9780879695767 (cloth) |
| Subjects |
Molecular cloning--Laboratory manuals ;
Cloning, Molecular--Laboratory Manuals
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| EKBM REF |
QH442.2.S26 2001 |
|
Research Triangle Park Library/RTP, NC |
03/07/2019 |
| EKCM |
QH442.2.S26 2001 |
|
CEMM/GEMMD Library/Gulf Breeze,FL |
08/07/2019 |
| ESBM |
QH442.2.S26 2001 |
|
CPHEA/PESD Library/Corvallis,OR |
09/19/2003 |
STATUS |
|
| Edition |
3rd ed. |
| Collation |
3 v. in 1 : ill. ; 28 cm. |
| Notes |
Includes bibliographical references and index. |
| Contents Notes |
Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning -- Ch. 2. Bacteriophage and its vectors -- Ch. 3. Working with bacteriophage M13 vectors -- Ch. 4. Working with high-capacity vectors -- Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Ch. 6. Preparation and analysis of eukaryotic genomic DNA -- Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells -- Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction -- Ch. 9. Preparation of radiolabeled DNA and RNA probes -- Ch. 10. Working with synthetic oligonucleotide probes -- Ch. 11. Preparation of cDNA libraries and gene identification -- Ch. 12. DNA sequencing -- Ch. 13. Mutagenesis -- Ch. 14. Screening expression libraries -- Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli -- Ch. 16. Introducing cloned genes into cultured mammalian cells -- Ch. 17. Analysis of gene expression in cultured mammalian cells -- Ch. 18. Protein interaction technologies. |
