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Main Title Comparison of the In vivo and In vitro Response of Mammalian Embryos to a Teratogenic Insult.
Author Warner, C. W. ; Sadler, T. W. ; Shockey, J. ; Smith, M. K. ;
CORP Author North Carolina Univ. at Chapel Hill. School of Medicine. ;Cincinnati Univ., OH.;Health Effects Research Lab., Cincinnati, OH.
Year Published 1983
Report Number EPA-R-808888; EPA/600/J-83/242;
Stock Number PB84-245505
Additional Subjects Toxicology ; In vivo analysis ; In vitro analysis ; Comparison ; Responses ; Reprints ; Teratogenesis ; Embryos ; Hydroxyurea
Library Call Number Additional Info Location Last
NTIS  PB84-245505 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
This study serves to further define the capabilities of the whole embryo culture system using the known teratogen, Hydroxyurea (HU). An initial in vivo study was performed where by day 9 pregnant mothers were injected IP with 300 mg/kg HU. Dams were sacrificed two days later and embryos were analyzed for malformations and total embryonic protein. In addition, the peak plasma value from injected dams was found to be approximately 300 micrograms/ml with a plasma half-life of 30 minutes. These values were then reproduced in the culture system with similar results noted in cultured embryos with respect to the type of malformations found. Additional in vitro experiments were performed varying both exposure time and drug level concentrations. Results indicate that both of these parameters are important considerations when designing in vitro experiments.