Record Display for the EPA National Library Catalog


Main Title Analysis of coprostanol, an indicator of fecal contamination /
Author Singley, J. E., ; Singley, J. Edward. ; Kirchmer, Cliff J. ; Miura., Ryosuke
Other Authors
Author Title of a Work
Kirchmer, Cliff Joseph,
Miura, Ryosuke,
CORP Author Florida Univ., Gainesville. Dept. of Environmental Engineering Sciences.;Environmental Protection Agency, Athens, Ga. Southeast Environmental Research Lab.
Publisher Office of Research and Development, U.S. Environmental Protection Agency, For sale by the Superintendent of Documents, U.S. Government Printing Office,
Year Published 1974
Report Number EPA 660/2-74-021; EPA-16020-EVG; W74-11794
Stock Number PB-235 491
OCLC Number 01834023
Subjects Cholesterol--Derivatives ; Sterols--Environmental aspects ; Organic water pollutants ; Cholestanol ; Coprostanol
Additional Subjects Coliform bacteria ; Feces ; Water analysis ; Biochemical oxygen demand ; Sewage treatment ; Chromatographic analysis ; Efficiency ; Colorimetric analysis ; Gas chromatography ; Cholestanols ; Water pollution detection ; Hydrazine/dinitrophenyl ; Chemical oxygen demand ; TOC(Total Organic Carbon)
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD  EPA 660-2-74-021 Headquarters Library/Washington,DC 06/09/2015
ELBD ARCHIVE EPA 660-2-74-021 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023
ELBD RPS EPA 660-2-74-021 repository copy AWBERC Library/Cincinnati,OH 11/13/2018
ERAD  EPA 660/2-74-021 Region 9 Library/San Francisco,CA 03/18/2013
NTIS  PB-235 491 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation xi, 114 pages : diagrams, graphs ; 27 cm.
Total coliform and fecal coliform have been used as measures of water quality for uses ranging from shellfish waters to public water supplies. Because of the weaknesses of the coliform tests, research has continued in the search for indicators of fecal pollution. Coprostanol, or 5 beta-cholestan-3 beta-ol, has shown promise as an indicator of fecal pollution. This sterol is a characteristic fecal organic compound which is found in the feces of higher animals, including man. The gas chromatographic analysis of coprostanol was improved and a method of sample preservation was developed using H2SO4. Field surveys compared coprostanol analyses with total and fecal coliform and confirmed the predicted advantages of a chemical method over a biological method. The gas chromatographic method was used in extensive field surveys, in degradation studies, in treatment plant efficiency studies and as the standard for evaluation of a colorimetric method. The method was capable of determining twenty nanograms per liter, which was estimated to be equivalent to approximately two coliforms per 100 ml. An attempt was made to correlate coprostanol concentration with treatment plant efficiency. There was a reasonably good correlation between coprostanol and BOD, COD and TOC. A colorimetric method has been developed that can be used to determine coprostanol at levels of one microgram g/l in polluted water. The color developer used is dinitrophenylhydrazine. (Modified author abstract)
Report prepared by Florida University, Department of Environmetal Engineering Sciences, Gainsville, Florida. Prepared for Office of Research and Development, U.S. Environmental Protection Agency. "EPA 660/2-74-021." "Project 16020 EVG; Program element 1BA027." Includes bibliographical references (pages 109-112).