| Main Title |
Keratinocyte Cell-Mediated Mutagenesis Assay: Correlation with In vivo Tumor Studies. |
| Author |
Reiners, Jr., J. J. ;
Yotti, L. P. ;
McKeown, C. K. ;
Nesnow, S. ;
Slaga, T. J. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Tennessee Univ., Oak Ridge. ;Oak Ridge National Lab., TN. |
| Year Published |
1983 |
| Report Number |
EPA/600/J-83/228; |
| Stock Number |
PB84-232719 |
| Additional Subjects |
Toxicology ;
In vivo analysis ;
Neoplasms ;
Reprints ;
Mutagenesis ;
Keratinocytes ;
Cell mediated analysis
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB84-232719 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
A murine keratinocyte cell-mediated mutagenesis assay was characterized and examined as an in vitro model system for studying the biotransformation of promutagens/procarcinogens by mouse skin. The assay used living cultured newborn SENCAR keratinocytes for the metabolic activation of promutagens and Chinese hamster lung V-79 fibroblasts for detection of resulting mutagens. Mutations at, or affecting, the hypoxanthineguanine phosphoribosyltransferease locus were scored by resistance to 6-thioguanine. The relative mutagenicities of several polycyclic aromatic hydrocarbons (PAHs) in the cell-mediated assay correlated with the in vivo skin tumorigenicity of the PAHs determined in a two-stage carcinogenesis protocol. |
