Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Detection of Fecal Coliforms in Water by Using ((14)C)Mannitol.
Author Reasoner, D. J. ; Geldreich, E. E. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/114;
Stock Number PB90-117110
Additional Subjects Coliform bacteria ; Aquatic microbiology ; Potable water ; Scintillation counters ; Spectrometers ; Graphs(Charts) ; Laboratory tests ; Reprints ; Drinking water ; Mannitol ; Microbiological technics
Library Call Number Additional Info Location Last
NTIS  PB90-117110 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/10/1990
Collation 7p
Interest in rapid bacterial detection methods for sanitary indicator bacteria in water prompted a study of the use of (14)C-mannitol (UL) to detect fecal coliforms (FC). A simple method was developed using m-FC broth, membrane filtration, and two-temperature incubation (35 C for 2 h, followed by 44.5 C for 2.5 h). Results indicated that FC numbers ranging from 1 x 10(1) to 2.1 x 10(5) cells could be detected in 4.5 h. Within sample reproducibility at all cell concentrations was good, but sample to sample reproducibility was variable. Comparisons between m-FC broth and m-FC broth modified by substituting 4-mannitol for lactose indicated that the standard m-FC broth was the better test medium. Results from experiments employing dimethyl sulfoxide (DMSO) to increase permeability of FC to (14)C-mannitol indicated no increase in (14)CO2 production due to DMSO. Detection of FC using this method may be useful for rapid analyses of potable water suspected of contamination due to distribution line breaks or cross-connections, or for quick surveys of potable source water quality.