Record Display for the EPA National Library Catalog


Main Title Seed germination and root elongation toxicity tests in hazardous waste site evaluation : methods development and applications /
Author Linder, Greg. ; Greene, J. C. ; Ratsch, H. ; Nwosu, J. ; Smith, S.
Other Authors
Author Title of a Work
Greene, Joseph C.
Ratsch, Hilman.
Nwosu, Julius.
Smith, Sheila.
Wilborn, David.
CORP Author Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR.
Publisher U.S. Environmental Protection Agency, Environmental Research Laboratory,
Year Published 1989
Report Number EPA/600/D-89/109; ERL-COR-1038D
Stock Number PB90-113184
Additional Subjects Toxicity ; Germination ; Plants(Botany) ; Hazardous materials ; Waste disposal ; Mutagenicity tests ; Soil pollutants ; Superfund program ; Environmental monitoring
Library Call Number Additional Info Location Last
NTIS  PB90-113184 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 34 pages ; 28 cm
Seed germination tests measure soil toxicity directly, while root elongation tests consider the indirect effects of water-soluble constituents which may be present in site-samples. In the seed germination toxicity test, site-soil is mixed with a reference soil to yield exposure concentrations into which test seeds are planted. Germination is evaluated after a five-day exposure, and effective concentrations associated with a 50% reduction in seed germination are calculated. Root elongation tests evaluate site-soil eluates. Here seeds are placed onto moistened filter paper which lines Petri dish exposure chambers; exposure chambers are then covered and incubated in complete darkness for five days. Inhibition of root elongation is calculated as an EC50 (exposure concentration which yields a 50% reduction in root length relative to controls) upon termination of the test.
"Paper presented at the First Symposium on Use of Plants in Toxicity Assessment, April 16-21, Atlanta, GA, 1989." Includes bibliographical references. "EPA/600/D-89/109"--Cover. Microfiche.