||Root-Mycocosm for Growing Ectomycorrhizal Hyphae Apart from Host Roots while Maintaining Symbiotic Integrity (Journal Version).
Rygiewicz, P. T. ;
Miller, S. L. ;
Durall, D. M. ;
||Corvallis Environmental Research Lab., OR. ;Oregon State Univ., Corvallis. Dept. of Forest Science.;National Science Foundation, Washington, DC.;Pacific Northwest Forest and Range Experiment Station, Corvallis, OR. Forestry Sciences Lab.
||EPA/600/J-88/233; NSF-BSR85-05975 ;NSF-BSR83-06181;
Gravimetric analysis ;
Plant growth ;
||Some EPA libraries have a fiche copy filed under the call number shown.
A culture system is described to grow mycorrhizal plants which allows experimental measurements to be made on mycorrhizae, and a portion of intact ectomycorrhizal fungi while in symbiosis, but growing apart from the rooting medium and host roots. A portion of the extramatrical hyphae is kept apart from the rooting medium by a restrictive passageway between the root and fungal chambers. Fungal hyphae grow out of the root chamber onto pre-weighted glass fiber filter paper for gravimetric estimation of the hyphal mass in the fungal chamber. The pieces of the modular Root-Mycocosm can be assembled in various configurations. Ponderosa pine (pinus ponderosa Laws.) seedlings were inoculated with Hebeloma crustuliniforme Quel in either commercial-vermiculite inoculum or in plastic growth-pouches and grown in the Root-Mycocosm. Hyphae were allowed to grow into the fungal chambers and after four weeks, amounted to 5.5 + or - 0.81 SE and 6.4 + or - 1.5 SE mg for pouch and commercial inoculum techniques, respectively. (Copyright 1988 Kluwer Academic Publishers.)