Record Display for the EPA National Library Catalog


Main Title Occurrence of 'Cryptosporidium' Oocysts in Sewage Effluents and Selected Surface Waters.
Author Madore, M. S. ; Rose, J. B. ; Gerba, C. P. ; Arrowood, M. J. ; Sterling, C. R. ;
CORP Author Arizona Univ., Tucson.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA-R-813536; EPA/600/J-87/212;
Stock Number PB88-169131
Additional Subjects Surface waters ; Sewage effluents ; Reprints ; Cryptospordium
Library Call Number Additional Info Location Last
NTIS  PB88-169131 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
An existing method for the detection of Cryptosporidium oocysts in water was modified to investigate oocyst prevalence in large volumes of water. Surface waters and sewage effluents were filtered, eluted from the filter, and concentrated using centrifugation. The resultant pellet was then homogenized, sonicated, and placed on a sucrose gradient to separate oocysts from the sediment. The uppermost gradient layer was then examined by immunofluorescence using a labeled monoclonal antibody. Using the technique, average numbers of oocysts detected in raw and treated sewage were 5.18 x 10 and 1.30 x 101/L, respectively. Filtered sewage effluents had significantly lower numbers of oocysts (10.0/L). These data show that sand filtration may reduce the concentrations of this parasite in waste waters. Highly variable oocyst numbers were encountered in surface waters. Since Cryptosporidium oocysts are frequently present in environmental waters, they could be responsible for waterborne outbreaks of disease.