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Main Title Direct Effects of Ethane Dimethanesulphonate on Epididymal Function in Adult Rats. An In vitro Demonstration.
Author Klinefelter, G. L. ; Roberts, N. L. ; Suarez, J. D. ;
CORP Author ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
Publisher c1992
Year Published 1992
Report Number EPA-68-02-4450; EPA/600/J-92/429;
Stock Number PB93-141281
Additional Subjects Toxicity ; Epididymis ; Rats ; In vitro analysis ; Spermatozoa ; Sperm motility ; Extracellular matrix ; Epithelium ; Cultured cells ; Electron microscopy ; Membrane proteins ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-141281 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 16p
It was recently demonstrated that the Leydig cell toxicant ethane dimethanesulphonate (EDS) produces multiple effects on the epididymis after a single in vivo exposure. To determine whether any of the perturbations were mediated by a direct action of the compound, we used a novel system for the coculture of epididymal epithelial cells and sperm from the caput epididymidis. This system maintains the morphologic integrity and cell polarity of the epididymal epithelial cells before and during coculture, and the sperm recovered after coculture have intact plasma and acrosomal membranes. In addition, several functions required for epididymal sperm maturation are expressed, including the secretion of protein by the epididymal epithelium, the association of secreted protein with the plasma membrane of cocultured sperm, and the acquisition of progressive motility by cocultured sperm. In vitro exposure of epididymal epithelial cells and sperm to EDS results in a significant decline in protein secretion by the epithelial cells during coculture, and in particular, a dose-dependent decline in a 36- to 38-kd protein (PI 4.0 to 4.5) and a 34- to 36-kd protein (PI 4.5 to 5.0). Moreover, these and other proteins are not recovered from the sperm membrane of cocultured sperm after EDS treatment. Finally, EDS results in a dose-dependent decline in the percentage of both motile and progressively motile sperm recovered after coculture compared with that of sperm from untreated cocultures. (Copyright (c) American Society of Andrology.)