Record Display for the EPA National Library Catalog


Main Title Effect of Glyphosate and Nitrapyrin on Selected Bacterial Populations in Continuous-Flow Culture.
Author Hendricks, C. W. ; Rhodes, A. N. ;
CORP Author Corvallis Environmental Research Lab., OR.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/401;
Stock Number PB93-121309
Additional Subjects Nitrogen fixing bacteria ; Nitrogen fixation ; Soil microbiology ; Herbicides ; Soil bacteria ; Fluorescent antibody technique ; Reprints ; Continuous flow culture ; Glyphosphate ; Nitrapyrin ; Cell enumeration ; Nitrosolobus ; Nitrosospira ; Nitrosomonas
Library Call Number Additional Info Location Last
NTIS  PB93-121309 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
The study examines the effects of Roundup (N-(phosphonomethyl) glycine) and N-Serve (2-chloro-6-(trichloromethyl) pyridine) on the culture of soil organisms in a continuous-flow column system. In the study, nitrifying and various heterotrophic bacterial populations were enumerated over a 16-day period. The chemicals Roundup and N-Serve were added at day 8. Heterotrophic bacterial populations increased significantly in columns with 4.2 mg nitrapyrin and 68 mg glyphosate per g dry soil. Numbers of heterotrophs were not significantly different from controls in columns at lower chemical concentrations. Numbers of nitrifying bacteria did not appear to change following treatment, although nitrification was inhibited. Fluorescent antibody analysis of the nitrifying bacteria revealed that Nitrosolobus was more numerous than Nitrosospira and Nitrosomonas. Nitrosolobus increased in number, whereas the other two genera remained unchanged. In the study, the continuous-flow system proved to be both reliable and useful in the culture of nitrifying bacteria. The method is an alternative to traditional techniques for evaluation of the effects of chemicals on microbial biogeochemical cycles.