Record Display for the EPA National Library Catalog


Main Title Continuous-Flow Method for Measuring Effects of Chemicals on Soil Nitrification.
Author Rhodes, A. N. ; Hendricks, C. W. ;
CORP Author Corvallis Environmental Research Lab., OR. ;Air Force Academy, CO.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/008;
Stock Number PB90-219668
Additional Subjects Chemical compounds ; Soil surveys ; Nutrients ; Microorganisms ; Nitrification ; Continuous sampling ; Plant growth ; Herbicides ; Site surveys ; Soil tests ; Nitrobacteraceae ; Statistical analysis ; Chemical analysis ; Reprints ; Terrestrial ecosystems ; Land pollution ; Roundup ; N-serve
Library Call Number Additional Info Location Last
NTIS  PB90-219668 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 16p
Because the productivity of terrestrial ecosystems is directly related to microbial nutrient cycling, understanding the effects of chemical contaminants on soil microbial processes is important. The study examined the effects of two model chemicals - Roundup (glyphosate) and N-Serve (nitrapyrin) - on nitrifying organisms in static, perfusion, and continuous-flow culture systems. Experimental concentrations were approximately 1, 10 and 100 X the spot application rate. Both products were shown to inhibit nitrification in the treated soils. Roundup significantly reduced nitrification at 6.8 and 68 mg/g dry soil. N-Serve completely inhibited nitrification at levels greater than 42 mg/g dry soil in all cultural methods. In comparative studies with static batch and perfusion culture techniques, the continuous-flow system proved to be both reliable and useful in the culture of nitrifying bacteria. The method provides an alternative to traditional culture techniques in measuring chemical effects on microbial geochemical cycles and provides a new method for use in toxicity testing.