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Main Title Laboratory procedure for determining substrate removal rate coefficients for biofilms /
Author Lewis, David L. ; Pope, J. D. ; Gattis, D. K. ; Said, W. A.
Other Authors
Author Title of a Work
Pope, John D.
Gattie, David K.
Sayed, William A.
CORP Author Environmental Research Lab., Athens, GA. ;Technology Applications, Inc., Athens, GA. ;Georgia Univ., Athens. Inst. of Ecology. ;Georgia State Univ., Atlanta.
Publisher U.S. Environmental Protection Agency Environmental Research Laboratory,
Year Published 1989
Report Number EPA 600/J-89/246
Stock Number PB90 137 084 National Technical Information Service
OCLC Number 43209714
Subjects Biofilms--Research
Additional Subjects Water microbiology ; Test methods ; Metabolism ; Substrates ; Mathematical models ; Reprints ; Biofilms ; Aquatic ecosystems ; Cell adhesion
Library Call Number Additional Info Location Last
ELBD RPS EPA 600-J-89-246 repository copy AWBERC Library/Cincinnati,OH 10/14/2020
NTIS  PB90-137084 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9 unnumbered pages : illustrations ; 28 cm
Although numerous techniques have been standardized for assessing the metabolic activities of free-living (suspended) microorganisms, little corresponding methodology has been developed for attached microorganisms. Because attached microorganisms, such as those in biofilms, may dominate the microbiology of shallow aquatic ecosystems, methods designed specifically for measuring their activities are a much needed tool in environmental science. The following method gives detailed procedures for measuring the rates of biofilm removal of rapidly degraded hydrophilic organic substrates. Using replicate Teflon strips colonized in aquatic microcosms and a benchmark chemical (2,4-dichlorophenoxyacetic acid methyl ester), substrate removal rate coefficients were determined for a particular ratio of colonized surface area to container volume. These coefficients are applicable in mathematical models used to predict chemical removal rates under field conditions. (Copyright (c) 1989 Elsevier Science Publishers B.V.)
Cover title. "EPA/600/J-89/246." Published in Journal of Microbiological Methods 10:2 (September 1989) ; 103-111. Includes bibliographical references (page 111).