Chambers are described that provide a unique capacity to study various aspects of plant physiology and learn the mechanisms and controlling factors for the uptake and distribution of chemicals in plants. The chambers have many of the features of smaller cuvettes, but allow study on larger, whole plants and plant canopies. Transpiration and photosynthesis are measured and can be controlled by altering the humidity and CO2 injection rate. The chambers provide for root as well as shoot studies in highly contaminated environments in which the environmental conditions are controlled and data is automatically collected. They are managed by an inexpensive computer and can be constructed at a reasonable cost.