Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Difference in Virulence of Environmental Isolates of 'Legionella pneumophila'.
Author Bollin, G. E. ; Plouffe, J. F. ; Para, M. F. ; Prior, B. ;
CORP Author Ohio State Univ., Columbus. Div. of Infectious Diseases.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA-R-812704 ;EPA-R-811023; EPA/600/J-85/468;
Stock Number PB87-170106
Additional Subjects Respiratory infections ; Potable water ; Hospitals ; Guinea pigs ; Laboratory animals ; Lethal dosage ; Dosage ; Reprints ; Legionnaires disease ; Virulence ; Environmental microbiology ; Legionella pneumophila
Library Call Number Additional Info Location Last
NTIS  PB87-170106 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 6p
Endemic nosocomial Legionnaires disease has occurred at Ohio State University Medical Center for several years. Two subtypes of Legionella pneumophila serogroup 1 (UH-1 and RH-1) have been isolated in approximately equal numbers from hospital potable water. However, almost all clinical isolates have been UH-1. To assess potential difference in virulence, 50% lethal doses (LD sub 50) and 50% infective doses (ID sub 50) of UH-1 and RH-1 were determined by intraperitoneal infection in guinea pigs. The UH-1 LD sub 50 was 7.41 x 1,000,000 CFU, which was significantly lower than the RH-1 LD sub 50 of 9.12 x 10 to the 7th power CFU (P = 0.0001). The mean time to death in UH-1-infected guinea pigs was also significantly shorter than in RH-1-infected animals (P=0.0008). The UH-1 ID sub 50 of 1.4 x 10,000 CFU, this difference was not statistically significant (P=0.21). This study demonstrates a difference in virulence between UH-1 and RH-1 in guinea pigs. Differences in strain virulence, as demonstrated between these two subtypes, may help to explain the widespread isolation of L. pneumophila from the environment contracted with the limited occurrence of human Legionnaires disease. (Copyright (c) 1985, American Society for Microbiology.)