Record Display for the EPA National Library Catalog


Main Title Acute Effects and Long-Term Sequelae of 1,3-Dinitrobenzene on Male Reproduction in the Rat. 1. Sperm Quality, Quantity, and Fertilizing Ability.
Author Linder, R. E. ; Hess, R. A. ; Perreault, S. D. ; Strader, L. F. ; Barbee, R. R. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc./Environmental Sciences, Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/376;
Stock Number PB90-103078
Additional Subjects Toxicology ; Fertility ; Reproduction(Biology) ; Males ; Rats ; Graphs(Charts) ; Reprints ; Spermatozoa ; Biological effects ; Dinitrobenzenes ; Organ weight
Library Call Number Additional Info Location Last
NTIS  PB90-103078 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Groups of eight adult male rats were given a single oral dose of 0 or 48 mg/kg of 1,3-dinitrobenzene and sacrificed at 1, 2, 4, 8, 16, 24, 32, 72, and 175 days posttreatment. The groups killed at 175 days were bred to untreated females during weeks 3, 4, 6, 9, 13, and 24. Decreased testis weight and testicular sperm numbers were observed by day 4; decreased cauda sperm reserves and epididymis weight occurred by day 8 and day 16, respectively. Reduced numbers of motile spermatozoa and abnormal sperm morphology were seen in spermatozoa from the cauda epididymidis by day 16. Fertilizing ability, as indicated by the presence of two pronuclei and a sperm tail in eggs flushed from the oviducts of inseminated females, was slightly reduced by week 4 and declined to zero by week 6. Group means for reproductive organ weights, sperm production, and sperm reserves failed to return to control levels although some individual animals approaching full recovery. Normal fertilizing ability was restored in most animals by week 13, but two of seven remained infertile.