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Main Title Ethane Dimethanesulfonate-Induced Decrease in the Fertilizing Ability of Cauda Epididymal Sperm Is Independent of the Testis.
Author Klinefelter, G. R. ; Laskey, J. W. ; Perreault, S. D. ; Ferrell, J. ; Jeffay, S. ;
CORP Author ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div.
Publisher cAug 94
Year Published 1994
Report Number EPA-68D20056; EPA/600/J-94/543;
Stock Number PB95-148052
Additional Subjects Toxicity ; Fertilization ; Spermatozoa ; Testis ; Epididymis ; Testosterone ; Orchiectomy ; Sperm motility ; Reprints ; Ethane dimethanesulfonate
Library Call Number Additional Info Location Last
NTIS  PB95-148052 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Several decades ago it was reported that when adult male rats were exposed to a single injection of 50 mg/kg body weight ethane dimethanesulfonate (EDS) and mated with untreated females, average litter size was significantly reduced as early as 2 weeks later. Herein, the authors sought to determine whether EDS perturbs the fertilizing ability of cauda epididymal sperm. Four days after exposure to 50 mg/kg EDS, sperm from the proximal cauda epididymidis were inseminated into adult receptive females in utero; on the next day the percentage of ferilized eggs was determined. Exogenous T administration and castraction were used to determine what role, if any, androgen deprivation and the testis had on the fertilizing ability of proximal cauda epididymal sperm. Sperm motion parameters, serum T, T in the caput/corpus epididymidis, and detergent-extracted sperm protein were evaluated and correlated with fertilizing ability. The authors found that both castration and EDS exposure significantly compromised the fertilizing ability of sperm in proximal cauda epididymidis 4 days after exposure. Exogenous T, sufficient to maintain serum T, completely restored the fertilizing ability of sperm following castration, but not after EDS exposure.