Record Display for the EPA National Library Catalog


Main Title Utility of Light Scatter in the Morphological Analysis of Sperm.
Author Zucker, R. M. ; Perreault, S. D. ; Elstein, K. H. ;
CORP Author ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Environmental Protection Agency, Research Triangle Park, NC.
Publisher c1992
Year Published 1992
Report Number EPA-68-02-4450; EPA/600/J-92/061;
Stock Number PB92-150754
Additional Subjects Spermatozoa ; Light scattering ; Cell morphology ; Flow cytometry ; Lasers ; Fluorescence ; Biophysics ; Animals ; Deoxyribonucleic acids ; Species specificity ; Refractivity ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-150754 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
A study differentiated the morphologically diverse sperm nuclei of four animal species by using an Ortho flow cytometer to detect the forward light scatter from a red (helium-neon) laser. Cytograms depicting the axial light loss and forward red scatter signals revealed unique, but reproducible, sigmoid distributions that reflected not only interspecies differences in shape and size, but variations in particle refractive index and orientation within the flow cell at the time of analysis. Consequently, the authors were able to use regional gating of the light scatter cytogram to minimize the influence of orientation on the resolution of the fluorescence signal. The authors also observed that sperm enlarging as a result of chemically induced decondensation exhibit over time a biphasic shift (increase, then decrease) in light scatter at a species-dependent rate. These results suggest that, without any special adaptations to the flow cytometer, light-scatter parameters can be used to discriminate morphologically different sperm, to enhance the resolution of fluorescence measurements that may otherwise be confounded by variability in radial orientation, and to detect alterations in the rate of a biochemical/biophysical process such as decondensation.