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Main Title Timing of Hamster Sperm Nuclear Decondensation and Male Pronucleus Formation Is Related to Sperm Nuclear Disulfide Bond Content.
Author Perreault, S. D. ; Naish, S. J. ; Zirkin, B. R. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Johns Hopkins Univ., Baltimore, MD. Dept. of Population Dynamics.
Year Published 1987
Report Number EPA/600/J-87/052;
Stock Number PB87-213245
Additional Subjects Germ cells ; Cell nucleus ; Chemical bonds ; Hamsters ; Time measurement ; Reproduction(Biology) ; Laboratory animals ; Cytology ; Reprints ; Disulfides ; Oocytes ; Epididymis
Library Call Number Additional Info Location Last
NTIS  PB87-213245 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
The relationship between the timing of both sperm nuclear decondensation and male pronucleus formation in the oocyte and the relative level of disulfide bonds within the sperm nucleus was evaluated. Four types of hamster sperm nuclei, in which the extent of S-S bonding differed, were microinjected into hamster oocytes, and the time course of sperm nuclear decondensation and male pronucleus formation was charted. Regardless of when decondensation occurred, formation of the male pronucleus never preceded that of the female pronucleus (which occurred 1.25-1.5 hours after microinjection). However, sperm nuclei with few S-S bonds were more likely than S-S rich nuclei to transform into male pronuclei in synchrony with the formation of the female pronucleus.