Record Display for the EPA National Library Catalog


Main Title Regulation of Sperm Nuclear Reactivation during Fertilization.
Author Perreault, S. D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher 1989
Year Published 1989
Report Number EPA/600/D-89/142;
Stock Number PB90-113200
Additional Subjects Germ cells ; Animal husbandry ; Fertility ; Deoxyribonucleic acids ; Protamines ; Histones ; Mammals ; Glutathione ; DNA replication ; Oocytes ; Species specificity ; Autoradiography
Library Call Number Additional Info Location Last
NTIS  PB90-113200 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Upon fusion of sperm and oocyte at fertilization, a series of events is initiated whereby the highly compacted sperm nucleus expands and is transformed into a male pronucleus capable of DNA synthesis. The regulation of these early post-fusion fertilization events has been studied using in vitro fertilization and sperm microinjection methods. It was learned that the first step in reactivation of the mammalian sperm nucleus is reduction of sperm protamine disulfide bonds by the oocyte. This step is modulated by glutathione, the levels of which increase during oocyte maturation and correlate with the relative sperm decondensing activity of oocytes at different developmental stages. Once sufficient protamine disulfide bonds have been released, the sperm chromatin becomes accessible to oocyte factors that induce its decondensation, which is dependent upon the type of protamine present, a species specific characteristic.