Record Display for the EPA National Library Catalog


Main Title Viable 'Legionella pneumophila' Not Detectable by Culture on Agar Media.
Author Hussong, D. ; Colwell, R. R. ; O'Brien, M. ; Weiss, E. ; Pearson, A. D. ;
CORP Author Maryland Univ., College Park. Dept. of Microbiology. ;Agricultural Research Service, Beltsville, MD. Soil-Microbial System Lab. ;Naval Medical Research Inst., Bethesda, MD. ;Central Public Health Lab., London (England). Communicable Disease Surveillance Centre.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1987
Report Number EPA-R-812246; EPA/600/J-87/348;
Stock Number PB88-220090
Additional Subjects Cultures(Biology) ; Microorganisms ; Tissue culture ; Bacteriology ; Reprints ; Legionella pneumophila ; Fluorescent antibody assay ; Legionnaires' disease ; Environmental samples
Library Call Number Additional Info Location Last
NTIS  PB88-220090 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
In the paper, environmental samples collected from sources implicated in an epidemic of Legionnaires' disease were surveyed. Although no cultures were recovered from environmental samples, numerous cells were observed by fluorescent microscopy when anti-L, pneumophila group 1 antibody was used. Similar observations have often been made by others. To study the loss of culturability, L. pneumophila strains were maintained in a microcosm (vessels containing sterilized environmental water) and assayed at intervals for growth on appropriate media, and lethality for chick embryos. At 4 deg C, the decimal rate of decline of colony forming cells was approximately 29 days; at 37 deg C it was 13 days. Results indicate that samples that do not contain culturable cells, may contain cells that are viable, as demonstrated by their pathogenicity for chick embryos. The fluorescent antibody assay may provide a valuable indication of the presence of such viable but non-culturable cells.