Record Display for the EPA National Library Catalog


Main Title Hypochlorite-Serum Reaction Products Inhibit Porcine Vascular Endothelial Cell Growth in Culture.
Author Papa, L. R. ; McCauley, P. T. ;
CORP Author Health Effects Research Lab., Cincinnati, OH.
Year Published 1985
Report Number EPA/600/J-85/242;
Stock Number PB86-137072
Additional Subjects Toxicology ; Chlorination ; Inhibitors ; In vitro analysis ; Blood cells ; Growth ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB86-137072 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
In vitro toxicity studies were initiated in order to determine if chlorination affects vascular endothelial cells. Twelfth to twentieth passage porcine aortic vascular endothelial cells (PAE) were grown to confluency and replated in the presence of complete media (Eagle's minimum essential media supplemented with 20% fetal bovine serum) which had been preincubated for 30 minutes with 15.0 mg/liter chlorine. During a 72-hour exposure period, control PAE cells grew to confluency, an increase of approximately 9 fold in the number of cells/plate. Those cells exposed to media preincubated with 15.0 mg/liter chlorine derived from sodium hypochlorite increased only 6 fold. There was no sign of cell killing, but an apparent inhibition of cell division. No effect was seen when either the amino acid or vitamin component of the complete media was reacted at the 15.0 mg dose level. However, when the serum component was preincubated with 15.0 mg chlorine/liter as sodium hypochlorite, an inhibition in growth rate similar to the complete media occurred.