Record Display for the EPA National Library Catalog


Main Title Dose-Response Analysis of Methoxychlor-Induced Alterations of Reproductive Development and Function in the Rat.
Author Gray, L. E. ; Ostby, J. ; Ferrell, J. ; Rehnberg, G. ; Linder, R. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/024;
Stock Number PB89-237481
Additional Subjects Toxicology ; Animal physiology ; DDT ; Males ; Females ; Rats ; Reproductive system ; Endocrinology ; Histology ; Reprints ; Dose-response relationships ; Sex maturation ; Biological effects
Library Call Number Additional Info Location Last
NTIS  PB89-237481 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 19p
Rats were dosed from weaning, through puberty and gestation, to Day 15 of lactation with methoxychlor at 25, 50, 100, or 200 mg/kg/day. Morphological landmarks of puberty were measured, including the ages at vaginal opening, first estrus, and first estrous cycle in females and at preputial separation in males. In the female, estrous cyclicity, fertility, litter size, number of implantation sites, organ weights, and ovarian and uterine histology were also measured. The viability of the offspring (F1) and their fertility were evaluated using a continuous breeding protocol. Males were necropsied after breeding, the reproductive organs were weighed, and the cauda epididymal sperm counts were determined. One testis was used for histopathology, while the other was used to quantify interstitial fluid (IF) content, IF testosterone concentration, and testicular sperm production. Testosterone and androgen-binding protein were measured in the caput epididymis, and sperm motility and morphology were evaluated from a caudal sample. The serum and pituitary were saved for hormonal determinations. Results are discussed. (Copyright (c) 1989 Society of Toxicology.)