Record Display for the EPA National Library Catalog


Main Title Co-Culture of Rat Embryos and Hepatocytes: 'In vitro' Detection of a Proteratogen.
Author Oglesby, L. A. ; Ebron, M. T. ; Carver, B. D. ; Kavlock, R. J. ; Beyer, P. E. ;
CORP Author Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA-68-02-4032; EPA/600/J-86/070;
Stock Number PB86-213766
Additional Subjects Toxicology ; In vitro analysis ; Rats ; Detection ; Embryos ; Hepatocytes ; Proteratogens
Library Call Number Additional Info Location Last
NTIS  PB86-213766 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Rat embryos removed from the dam on day 10 of pregnancy were successfully co-cultivated in vitro with primary cultures of rat, rabbit, or hamster hepatocytes. Embryos co-cultivated with hepatocytes developed normally, as did embryos exposed to a test chemical, cyclophosphamide. In a system where embryos were co-cultivated with hepatocytes and then exposed to cyclophosphamide a dose-related embryotoxicity was observed, indicating metabolic activation of the proteratogen. When similar numbers of hepatocytes were used for metabolic activation of cyclophosphamide, the induced embryo-toxicity was greater in the presence of rabbit and hamster hepatocytes than with rat hepatocytes.