||Construction of Plasmids for Use in Risk Assessment Research.
Zylstra, G. J. ;
Cuskey, S. M. ;
Olsen, R. H. ;
||Michigan Univ., Ann Arbor. Medical School.;Environmental Research Lab., Gulf Breeze, FL.
Public health ;
Deoxyribonucleic acids ;
Safety engineering ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The report describes a series of selftransmissible and nonselftransmissible (cloning vector) plasmids constructed to compare results from different laboratory tests and plasmid systems. Plasmids were designed to overcome problems of reproducibility, confusion due to use of different genetic structures with a wide range of capabilities, and background contamination when enumerating released organisms or recipients in gene transfer determinations. Inclusion of one or more such plasmids may alleviate some ambiguity in analysis of data from different environmental milieus.