Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Development of an Intact Hepatocyte Activation System for Routine Use with the Mouse Lymphoma Assay.
Author Brock, K. H. ; Moore, M. M. ; Oglesby, L. A. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/J-87/194;
Stock Number PB88-165998
Additional Subjects Hepatocytes ; Lymphoma ; In viro analysis ; Mice ; Rats ; Cell cultures ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB88-165998 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 13p
The authors have developed a method for cocultivating primary rat hepatocytes with L5178Y/TK+/- 3.7.2C mouse lymphoma cells. The system should provide a means to simulate more closely in vivo metabolism compared to metabolism by liver homogenates, while still being useful for routine screening. Hepatocytes were isolated from 200-250 g adult male Sprague-Dawley rats. Rapid attachment of the hepatocytes (2 h) was obtained by using fibronectin-coated 25-cm2 tissue culture flasks. Several factors proved important in obtaining optimal cocultivated mouse lymphoma cell growth. These included maintaining the cocultivated cultures on a rocker platform instead of stationary and either (1) reducing the cocultivation time from 16 to 4 h or (2) utilizing less than 1 x 10 to the 6th power hepatocytes per flask for 16-h exposures. CP, DMN, DMBA, and B(a)P were used to demonstrate the capability of the system to detect mutagens that require metabolic activation.