||Co-Culture of Rat Embryos and Hepatocytes: 'In vitro' Detection of a Proteratogen.
Oglesby, L. A. ;
Ebron, M. T. ;
Carver, B. D. ;
Kavlock, R. J. ;
Beyer, P. E. ;
||Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
In vitro analysis ;
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Rat embryos removed from the dam on day 10 of pregnancy were successfully co-cultivated in vitro with primary cultures of rat, rabbit, or hamster hepatocytes. Embryos co-cultivated with hepatocytes developed normally, as did embryos exposed to a test chemical, cyclophosphamide. In a system where embryos were co-cultivated with hepatocytes and then exposed to cyclophosphamide a dose-related embryotoxicity was observed, indicating metabolic activation of the proteratogen. When similar numbers of hepatocytes were used for metabolic activation of cyclophosphamide, the induced embryo-toxicity was greater in the presence of rabbit and hamster hepatocytes than with rat hepatocytes.