This manual and its companion documents describe a three phase approach to provide guidance to dischargers in identifying toxicity in municipal and industrial effluents (Phase I, EPA, 1991A; EPA, 1992; and Phase III, EPA, 1993A). In 1989, the document titled 'Methods for Aquatic Toxicity Identification Evaluations: Phase II Toxicity Identification Procedures' was published as a guidance document for identifying the cause of toxicity in acutely toxic effluents (EPA, 1989A). This new Phase II document provides details for more types of samples, tests and test procedures that can be used to identify the specific chemical(s) responsible for acute or chronic effluent toxicity when the cause of toxicity is related to non-polar organic compounds, ammonia, surfactants, chlorine, or metals. Phase I characterization and Phase III confirmation, the other manuals in the three phase toxicity identification evaluations (TIE) approach, have also been produced or updated to include chronic toxicity information, and new developments made since the first set of documents were printed. The TIE-approach is applicable to effluents, ambient waters, sediment pore waters or elutriates, and hazardous waste leachates.

ASci Corporation Contract Number: contract no. 68-C0-0058 "September 1993." "Previous phase II methods by D.I. Mount and L. Anderson-Carnahan, EPA-600/3-88/035." "EPA-600/R-92/080." Includes bibliographical references.