Record Display for the EPA National Library Catalog


Main Title Urban Aerosol Acids: Analysis of National Institute of Standards and Technology Standard Reference Material 1649.
Author Sparacino, C. M. ; Frazier, S. E. ; Nishioka, M. G. ; Lewtas, J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Research Triangle Inst., Research Triangle Park, NC. ;Battelle, Columbus, OH.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/542;
Stock Number PB91-242529
Additional Subjects Aerosols ; Fractionation ; Particulates ; Air pollution ; Bioassay ; Samples ; Urban areas ; Mutagenicity tests ; Chemical analysis ; Organic compounds ; Dichlorophen ; Fungicides ; Reprints ; SRM 1649 ; Standard reference materials
Library Call Number Additional Info Location Last
NTIS  PB91-242529 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 16p
Urban air particulate matter, collected from Washington, D.C. and certified by the National Institute of Standards and Technology (NIST) as Standard Reference Material 1649, was extracted and fractionated into acid, base and neutral fractions. Each fraction was tested for biological activity using a microbial mutagenesis assay system. The organic acid fraction showed unexpectedly high mutagenic activity, and was subjected to chemical characterization studies. Following derivatization, analysis by GC/MS showed the presence of fatty acids, aromatic acids (including phenolic compounds), and a significant number of compounds that could not be identified from mass spectral compendia. Spectroscopic and elemental analysis data supported the characterization of the fraction as predominantly aromatic. Mass spectra from both GC/MS and direct probe analysis showed the presence of a chlorinated substance, subsequently identified as the fungicide Dichlorophen. The compound was shown to comprise over 50 percent of the mass of the organic acid fraction. A reference standard of Dichlorophen was not mutagenic. The presence of the fungicide in the NIST certified urban aerosol is, in all probability due to artifactual processes. Attempts to concentrate the observed mutagenic activity by preparative chromatography and acid/base partition experiments were not successful. (Copyright (c) 1990 Gordon and Breach, Science Publishers, Inc.)