Record Display for the EPA National Library Catalog

RECORD NUMBER: 40 OF 67

Main Title Morphological Transformation and DNA Adduct Formation by Benz(j)aceanthrylene and Its Metabolites in C3H10T1/2CL8 Cells: Evidence for Both Cyclopenta-Ring and Bay-Region Metabolic Activation Pathways.
Author Nesnow, S. ; Lasley, J. ; Curti, S. ; Ross, J. ; Nelson, G. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;Environmental Protection Agency, Research Triangle Park, NC. Carcinogenesis and Metabolism Branch.
Publisher c1991
Year Published 1991
Report Number EPA-68-02 4456; EPA/600/J-92/062;
Stock Number PB92-150762
Additional Subjects Mutagens ; DNA adducts ; Metabolism ; Aromatic polycyclic hydrocarbons ; Cultured cells ; Metabolic activation ; Phosphorus 32 ; Cell survival ; Cell morphology ; Thin layer chromatography ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified		 Checkout
Status
NTIS  PB92-150762 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Abstract
Benz(j)aceanthrylene (B(j)A), a cyclopenta-fused polycyclic aromatic hydrocarbon related to 3-methylcholanthrene, has been studied to identify the major routes of metabolic activation in transformable C3H10T1/2CL8(C3H10T1/2) mouse embryo fibroblasts in culture. Previous studies have reported that the major (55% of total) B(j)A metabolite formed by C3H10T1/2 cells was (+ or -)-trans-9,10-dihydroxy-B(j)A(B(jA-9,10-diol), the dihydrodiol in the bay-region ring, with moderate amounts (14% of total) of (+ or -)-trans-1,2-dihydro-1,2-dihydroxy-B(j)A (B(j)A-1,2-diol), the cyclopenta-ring dihydrodiol. Qualitative and quantitative analysis of the postlabeling data suggests that B(j)A is metabolically activated by two distinct routes, the bay-region diol-epoxide route and the cyclopenta-ring oxide route, the former being the most significant.