Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Comparison of Antioxidant Substances in Bronchoalveolar Lavage Cells and Fluid from Humans, Guinea Pigs, and Rats.
Author Slade, R. ; Crissman, K. ; Norwood, J. ; Hatch, G. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Environmental Toxicology Div.
Publisher 1993
Year Published 1993
Report Number EPA/600/J-94/020;
Stock Number PB94-137346
Additional Subjects Antioxidants ; Bronchoalveolar lavage fluid ; Species diversity ; Cells(Biology) ; Comparison ; Humans ; Guinea pigs ; Rats ; Proteins ; Lipids ; Air pollutants ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-137346 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 05/14/1994
Collation 17p
Antioxidants located in the lining layer of the respiratory tract may be important in determining sensitivity of lung tissues to inhaled pollutants. This study addressed species differences in the amounts of ascorbic acid (AH2), glutathione (GSH), uric acid (UA), and alpha-tocopherol (AT) in bronchoalveolar lavage (BAL) fluid and cells of humans, guinea pigs, and rats. Protein and lipid phosphorus (lipid P) were used as normalizing factors. More than 90% of the lavageable AH2, UA, GSH, protein, and lipid P was present in the extracellular fraction of BAL in rats and guinea pigs, while over 95% of the lavageable AT was located in the BAL cells. BAL fluid AH2/protein in rats was 7- to 9-fold higher than in humans and guinea pigs. However, human BAL fluid had 2- to 8-fold higher UA/protein. GSH/protein, and AT/protein ratios than rats and guinea pigs. In BAL cells, rats had higher AH2/protein and AT/protein ratios than guinea pigs and humans, and both rats and guinea pigs had higher GSH and AT/protein ratios than humans. Individual variability among humans in the BAL fluid and cellular antioxidants was generally greater than in the laboratory animals. These data demonstrate that some large species differences exist in BAL fluid and cellular antioxidants which could affect susceptibility to oxidant pollutants.