1. The essence of metabolic engineering -- 2. Review of cellular metabolism -- 3. Comprehensive models for cellular reactions -- 4. Material balances and data consistency -- 5. Regulation of metabolic pathways -- 6. Examples of pathway manipulations : metabolic engineering in practice -- 7. Metabolic pathway synthesis -- 8. Metabolic flux analysis -- 9. Methods for the experimental determination of metabolic fluxes by isotope labeling -- 10. Applications of metabolic flux analysis -- 11. Metabolic control analysis -- 12. Analysis of structure of metabolic networks -- 13. Flux analysis of metabolic networks -- 14. Thermodynamics of cellular processes. Metabolic engineering is an emerging, interdisciplinary field with applications to the production of chemicals, fuels, materials, and pharmaceuticals. The field's novelty lies in the integration of the techniques of molecular biology with the tools of mathematical analysis, to help elucidate metabolic flux control and rational selection of targets for genetic modification. By providing a rigorous description of cell physiology, metabolic engineering also facilitates functional genomics. Its primary objective is to identify specific genetic and environmental manipulations that lead to enhancement of yield and productivity of biotechnological processes, or the overall improvement of cellular properties.