Record Display for the EPA National Library Catalog


Main Title In-vitro methods for evaluating side effects of pesticides and toxic substances /
Author Narahashi, Toshio.
CORP Author Duke Univ. Medical Center, Durham, N.C. Dept. of Physiology and Pharmacology.;Health Effects Research Lab., Research Triangle Park, N.C.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Health Effects Research Laboratory ; Distributed by National Technical Information Service,
Year Published 1976
Report Number EPA-600/1-76-035; EPA-68-02-1289
Stock Number PB 260 385
OCLC Number 08370892
Subjects Insecticides--toxicity ; Receptors, Adrenergic--drug effects ; Receptors, Cholinergic--drug effects
Additional Subjects Pesticides ; Toxicology ; Insecticides ; Toxicity ; In vitro analysis ; Tissues(Biology) ; Adrenergics ; Cholinergics ; Carbamates ; Phosphorus organic compounds ; Evaluation ; Cholinesterase inhibitors ; Toxic substances ; Receptors
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD  EPA 600-1-76-035 c.1 Headquarters Library/Washington,DC 03/21/2014
EKBD  EPA-600/1-76-035 Research Triangle Park Library/RTP, NC 02/23/2000
EKCD  EPA-600/1-76-035 CEMM/GEMMD Library/Gulf Breeze,FL 05/29/2018
ELBD ARCHIVE EPA 600-1-76-035 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023 DISPERSAL
NTIS  PB-260 385 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation v, 120 [i.e. 64] pages : illustrations ; 28 cm.
Several skeletal muscle and smooth muscle preparations have been examined for their usefulness in evaluating the toxic effects of a variety of insecticides. The following preparations were found satisfactory for such test: guinea pig ileum for muscarinic receptors, guinea pig heart for beta-adrenergic receptors, guinea pig vas deferens for alpha-adrenergic receptors, frog rectus abdominis for nicotinic receptors of tonic muscle, and rat diaphragm for nicotinic receptors of phase muscle. Five carbamate insecticides, four organophosphate insecticides and chlordimeform were studied. None of the insecticides tested had any direct and potent effect on these receptors except the effect on cholinergic receptors via cholinesterase inhibition. Carbofuran, propoxur and formetanate had potent stimulating actions on the guinea pig ileum, but these effects could entirely be attributed to the accumulation of acetylcholine in the synaptic cleft as a result of cholinesterase inhibition. Thus, it can be concluded that these insecticides exert no direct action on cholinergic and adrenergic receptors.
"EPA-600/1-76-035." "Contract no. 68-02-1289." "November 1976." Includes bibliographical references (page 28). Photocopy.